Format

Send to

Choose Destination
J Cell Sci. 2018 Aug 10;132(4). pii: jcs218321. doi: 10.1242/jcs.218321.

Lipid-dependence of target membrane stability during influenza viral fusion.

Author information

1
Section on Integrative Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA.
2
Section on Integrative Biophysics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD 20892, USA joshz@helix.nih.gov.

Abstract

Although influenza kills about a half million people each year, even after excluding pandemics, there is only one set of antiviral drugs: neuraminidase inhibitors. By using a new approach utilizing giant unilamellar vesicles and infectious X-31 influenza virus, and testing for the newly identified pore intermediate of membrane fusion, we observed ∼30-87% poration, depending upon lipid composition. Testing the hypothesis that spontaneous curvature (SC) of the lipid monolayer controls membrane poration, our Poisson model and Boltzmann energetic considerations suggest a transition from a leaky to a non-leaky fusion pathway depending on the SC of the target membrane. When the target membrane SC is below approximately -0.20 nm-1 fusion between influenza virus and target membrane is predominantly non-leaky while above that fusion is predominantly leaky, suggesting that influenza hemagglutinin (HA)-catalyzed topological conversion of target membranes during fusion is associated with a loss of membrane integrity.

KEYWORDS:

Fusion; Membrane; Poration; Spontaneous curvature; Virus

PMID:
29967032
PMCID:
PMC6398481
[Available on 2020-02-15]
DOI:
10.1242/jcs.218321
Free full text

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center