Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell's Th17 response

Zhoutao He; Xiaoning Sun; Zhichao Ma; Jiao Fu; Baili Huang; Fujin Liu; Yi Chen; Taozhi Deng; Xiangyang Han; Deming Sun; Cheng Lan

doi:10.1186/s13578-018-0237-z

Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell's Th17 response

Cell Biosci. 2018 Jun 5:8:38. doi: 10.1186/s13578-018-0237-z. eCollection 2018.

Authors

Zhoutao He¹, Xiaoning Sun¹, Zhichao Ma², Jiao Fu², Baili Huang², Fujin Liu³, Yi Chen¹, Taozhi Deng¹, Xiangyang Han¹, Deming Sun⁴, Cheng Lan¹

Affiliations

¹ 1Department of Gastroenterology, Hainan General Hospital, Xiu Hua Road 19th, Xiu Ying District, Haikou, 570311 Hainan Province China.
² 2Medical Research Center, Hainan General Hospital, Haikou, 570311 Hainan Province China.
³ 3Department of Phathology, Hainan General Hospital, Haikou, 570311 Hainan Province China.
⁴ 4Doheny Eye Institute, Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90033 USA.

Abstract

Background: This study investigated the role of HSP70 in modulating intestinal γδ T cells' Th17 response in Trichinella spiralis-induced PI-IBS mice model.

Methods: The intestinal HSP70's expression and mRNA level were measured by Western blot and RT-PCR. The intestinal γδ T cell's morphological changes were analyzed using immunofluorescence staining and confocal laser scanning microscope. The pro-inflammatory cytokines' level was detected by ELISA. The isolated and purified γδ T cells were pre-incubated with HSP70 and their functions including proliferation, apoptosis, activation and production of IL-17 were also detected.

Results: Heat treatment augmented intestinal HSP70 expression and alleviated the clinical presentations in PI-IBS mice. Meanwhile, intestinal γδ T cells and local IL-17 level were increased by pre-induction of HSP70. HSP70 promoted the proliferation of PI-IBS mice's intestinal γδ T cells, inhibited the apoptosis and stimulated these cells to secret IL-17 rather than IFN-γ.

Conclusion: Our results suggest that HSP70 plays a protective role via up-regulating intestinal γδ T cell's Th17 response in PI-IBS mice.

Keywords: Heat shock protein 70; Mice; Post-infectious irritable bowel syndrome; Th17 response; γδ T cells.