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Methods Mol Biol. 2018;1801:265-285. doi: 10.1007/978-1-4939-7902-8_21.

De Novo Transcriptomic Approach to Study Thyroid Hormone Receptor Action in Non-mammalian Models.

Author information

1
Function and Mechanism of Action of Thyroid Hormone Receptor group, UMR 7221 CNRS and Muséum National d'Histoire Naturelle, Sorbonne Universités, Paris, France.
2
Function and Mechanism of Action of Thyroid Hormone Receptor group, UMR 7221 CNRS and Muséum National d'Histoire Naturelle, Sorbonne Universités, Paris, France. sachs@mnhn.fr.

Abstract

Thyroid hormones are pleiotropic hormones involved in chordates physiology. Understanding their functions and mechanisms is also instrumental to diagnose dys-regulations and get a predictive power that can applied to medicine, ecology, etc. Today, high-throughput sequencing technologies offer the opportunity to address this issue not only in model organisms but also in non-model organisms. Here, we describe a method that makes use of RNA-seq to address differential expression analysis in non-model organism.

KEYWORDS:

Differential expression analysis; Thyroid hormones; Transcriptome annotation; Transcriptome assembly

PMID:
29892831
DOI:
10.1007/978-1-4939-7902-8_21
[Indexed for MEDLINE]

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