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Nat Biotechnol. 2018 Jul;36(6):530-535. doi: 10.1038/nbt.4140. Epub 2018 May 28.

Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system.

Author information

1
Institute of Biological Interfaces and Department of Chemistry, Sogang University, Seoul, Korea.
2
Disease Biophysics Group, Wyss Institute for Biologically Inspired Engineering, John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA.
3
Department of Energy Science, Sungkyunkwan University, Suwon, Korea.
4
Sogang-Harvard Research Center for Disease Biophysics, Sogang University, Seoul, Korea.
5
Department of Life Science and Institute of Biological Interfaces, Sogang University, Seoul, Korea.
6
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA.
7
Department of Organismic and Evolutionary Biology, Department of Physics, Wyss Institute for Biologically Inspired Engineering, Kavli Institute for Nanobio Science and Technology, Harvard University, Cambridge, Massachusetts, USA.

Abstract

Inside cells, complex metabolic reactions are distributed across the modular compartments of organelles. Reactions in organelles have been recapitulated in vitro by reconstituting functional protein machineries into membrane systems. However, maintaining and controlling these reactions is challenging. Here we designed, built, and tested a switchable, light-harvesting organelle that provides both a sustainable energy source and a means of directing intravesicular reactions. An ATP (ATP) synthase and two photoconverters (plant-derived photosystem II and bacteria-derived proteorhodopsin) enable ATP synthesis. Independent optical activation of the two photoconverters allows dynamic control of ATP synthesis: red light facilitates and green light impedes ATP synthesis. We encapsulated the photosynthetic organelles in a giant vesicle to form a protocellular system and demonstrated optical control of two ATP-dependent reactions, carbon fixation and actin polymerization, with the latter altering outer vesicle morphology. Switchable photosynthetic organelles may enable the development of biomimetic vesicle systems with regulatory networks that exhibit homeostasis and complex cellular behaviors.

PMID:
29806849
DOI:
10.1038/nbt.4140
[Indexed for MEDLINE]

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