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Nat Commun. 2018 Apr 23;9(1):1604. doi: 10.1038/s41467-018-03921-6.

A B-ARR-mediated cytokinin transcriptional network directs hormone cross-regulation and shoot development.

Author information

1
Plant Biology Laboratory, and Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
2
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
3
Department of Computer Science, Dartmouth College, Hanover, NH, 03755, USA.
4
The Razavi Newman Integrative Genomics and Bioinformatics Core Facility, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA.
5
Bioinformatics Program, University of California at San Diego, La Jolla, CA, 92093, USA.
6
Plant Biology Laboratory, and Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA. ecker@salk.edu.
7
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, 92037, USA. ecker@salk.edu.

Abstract

Cytokinin fulfills its diverse roles in planta through a series of transcriptional responses. We identify the in vivo DNA binding site profiles for three genetically redundant type-B ARABIDOPSIS RESPONSE REGULATORS (B-ARRs): ARR1, ARR10, and ARR12. The expression and genome-wide DNA binding locations of the three B-ARRs extensively overlap. Constructing a primary cytokinin response transcriptional network reveals a recurring theme of widespread cross-regulation between the components of the cytokinin pathway and other plant hormone pathways. The B-ARRs are found to have similar DNA binding motifs, though sequences flanking the core motif were degenerate. Cytokinin treatments amalgamate the three different B-ARRs motifs to identical DNA binding signatures (AGATHY, H(a/t/c), Y(t/c)) which suggests cytokinin may regulate binding activity of B-ARR family members. Furthermore, we find that WUSCHEL, a key gene required for apical meristem maintenance, is a cytokinin-dependent B-ARR target gene, demonstrating the importance of the cytokinin transcription factor network in shoot development.

PMID:
29686312
PMCID:
PMC5913131
DOI:
10.1038/s41467-018-03921-6
[Indexed for MEDLINE]
Free PMC Article

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