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ACS Synth Biol. 2018 May 18;7(5):1195-1200. doi: 10.1021/acssynbio.8b00003. Epub 2018 Apr 18.

Bioprinting Living Biofilms through Optogenetic Manipulation.

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Department of Biomedical Engineering, College of Life Science and Technology , Huazhong University of Science and Technology , Wuhan 430074 , PR China.
Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, CAS Key Laboratory of Soft Matter Chemistry , University of Science and Technology of China , Hefei 230026 , PR China.


In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living bacteria to autonomically form patterned biofilms following prescribed illumination. The results showed that through optogenetic manipulation, patterned bacterial communities with high spatial resolution (approximately 10 μm) could be constructed in 6 h. Thus, optogenetic manipulation greatly increases the range of available bioprinting techniques.


Pseudomonas aeruginosa; bacterial biofilms; bioprinting; optogenetic manipulation


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