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J Med Primatol. 2018 Jun;47(3):157-171. doi: 10.1111/jmp.12340. Epub 2018 Mar 30.

The non-human primate kidney transcriptome in fetal development.

Author information

1
Department of Internal Medicine, Wake Forest University Health Sciences, Winston-Salem, NC, USA.
2
Department of Genetics, Texas Biomedical Research Institute, San Antonio, TX, USA.
3
Pharmaceuticals and Bioengineering Department, Southwest Research Institute, San Antonio, TX, USA.
4
Department of Pathology, University of Texas Health Science Center, San Antonio, TX, USA.
5
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio, TX, USA.
6
Department of Animal Science, University of Wyoming, Laramie, WY, USA.
7
Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.

Abstract

BACKGROUND:

Little is known about the repertoire of non-human primate kidney genes expressed throughout development. The present work establishes an understanding of the primate renal transcriptome during fetal development in the context of renal maturation.

METHODS:

The baboon kidney transcriptome was characterized at 60-day gestation (DG), 90 DG, 125 DG, 140 DG, 160 DG and adulthood (6-12 years) using gene arrays and validated by QRT-PCR. Pathway and cluster analyses were used to characterize gene expression in the context of biological pathways.

RESULTS:

Pathway analysis indicated activation of pathways not previously reported as relevant to kidney development. Cluster analysis also revealed gene splice variants with discordant expression profiles during development.

CONCLUSIONS:

This study provides the first detailed genetic analysis of the developing primate kidney, and our findings of discordant expression of gene splice variants suggest that gene arrays likely provide a simplified view and demonstrate the need to study the fetal renal proteome.

KEYWORDS:

array; baboon; cluster analysis; gene expression; ontogeny; pathway analysis

PMID:
29603257
PMCID:
PMC5963710
[Available on 2019-06-01]
DOI:
10.1111/jmp.12340

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