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Methods Mol Biol. 2018;1753:167-177. doi: 10.1007/978-1-4939-7720-8_11.

Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease.

Author information

1
Neurobiology-Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA. yogita@nbrc.ac.in.
2
Department of Cellular and Molecular Neuroscience, National Brain Research Centre, Manesar, Haryana, India. yogita@nbrc.ac.in.
3
Neurobiology-Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD, USA. swaroopa@nih.gov.

Abstract

Mitochondrial dysfunction, and consequently altered aerobic energy metabolism, is associated with numerous retinal diseases, including photoreceptor degeneration and diabetic retinopathy. Here, we describe a detailed protocol to directly measure oxygen consumption in the intact retina ex vivo using microplate-based fluorescence technology. We have used this method to assess preferred energy substrate for retinal tissue and suggested its application for investigating mechanisms of retinal disease.

KEYWORDS:

Aerobic respiration; Energy metabolism; Mouse models; Photoreceptor; Retinal degeneration

PMID:
29564788
DOI:
10.1007/978-1-4939-7720-8_11
[Indexed for MEDLINE]

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