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Insect Biochem Mol Biol. 2018 Apr;95:1-9. doi: 10.1016/j.ibmb.2018.03.001. Epub 2018 Mar 8.

A deep insight into the male and female sialotranscriptome of adult Culex tarsalis mosquitoes.

Author information

1
Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway Room 2E32D, Rockville, MD, 20852, United States. Electronic address: jribeiro@niaid.nih.gov.
2
Section of Vector Biology, Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, 12735 Twinbrook Parkway Room 2E32D, Rockville, MD, 20852, United States.
3
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Dr., Madison, WI, 53706, United States.

Abstract

Previously, a Sanger-based sialotranscriptome analysis of adult female Culex tarsalis was published based on ∼2000 ESTs. During the elapsed 7.5 years, pyrosequencing has been discontinued and Illumina sequences have increased considerable in size and decreased in price. We here report an Illumina-based sialotranscriptome that allowed finding the missing apyrase from the salivary transcriptome of C. tarsalis, to determine several full-length members of the 34-62 kDa family, when a single EST has been found previously, in addition to identifying many salivary families with lower expression levels that were not detected previously. The use of multiple libraries including salivary glands and carcasses from male and female organisms allowed for an unprecedented insight into the tissue specificity of transcripts, and in this particular case permitting identification of transcripts putatively associated with blood feeding, when exclusive of female salivary glands, or associated with sugar feeding, when transcripts are found upregulated in both male and female glands.

KEYWORDS:

Blood-feeding; Mosquitoes; Salivary glands; Transcriptome

PMID:
29526772
PMCID:
PMC5927831
DOI:
10.1016/j.ibmb.2018.03.001
[Indexed for MEDLINE]
Free PMC Article

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