Send to

Choose Destination
Anal Chem. 2018 Apr 3;90(7):4832-4839. doi: 10.1021/acs.analchem.8b00298. Epub 2018 Mar 19.

Time-Gated Raman Spectroscopy for Quantitative Determination of Solid-State Forms of Fluorescent Pharmaceuticals.

Author information

Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy , University of Helsinki , Viikinkaari 5 E , FI-00790 Helsinki , Finland.
Department of Future Technologies , University of Turku , Vesilinnantie 5 , FI-20500 Turku , Finland.
Nanoscience Center, Department of Chemistry , University of Jyväskylä , P.O. Box 35, FI-40014 , Jyväskylä , Finland.
TimeGate Instruments , Teknologiantie 5 , FI-90590 Oulu , Finland.


Raman spectroscopy is widely used for quantitative pharmaceutical analysis, but a common obstacle to its use is sample fluorescence masking the Raman signal. Time-gating provides an instrument-based method for rejecting fluorescence through temporal resolution of the spectral signal and allows Raman spectra of fluorescent materials to be obtained. An additional practical advantage is that analysis is possible in ambient lighting. This study assesses the efficacy of time-gated Raman spectroscopy for the quantitative measurement of fluorescent pharmaceuticals. Time-gated Raman spectroscopy with a 128 × (2) × 4 CMOS SPAD detector was applied for quantitative analysis of ternary mixtures of solid-state forms of the model drug, piroxicam (PRX). Partial least-squares (PLS) regression allowed quantification, with Raman-active time domain selection (based on visual inspection) improving performance. Model performance was further improved by using kernel-based regularized least-squares (RLS) regression with greedy feature selection in which the data use in both the Raman shift and time dimensions was statistically optimized. Overall, time-gated Raman spectroscopy, especially with optimized data analysis in both the spectral and time dimensions, shows potential for sensitive and relatively routine quantitative analysis of photoluminescent pharmaceuticals during drug development and manufacturing.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center