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Development. 2018 Feb 23;145(4). pii: dev155275. doi: 10.1242/dev.155275.

Connexin 30 controls astroglial polarization during postnatal brain development.

Author information

1
Center for Interdisciplinary Research in Biology, Collège de France, CNRS UMR 7241, INSERM U1050, Labex Memolife, PSL Research University, Paris 75005, France.
2
Doctoral School N°158, Pierre and Marie Curie University, Paris 75005, France.
3
Doctoral School N°562, Paris Descartes University, Paris 75006, France.
4
Institut Pasteur, CNRS UMR 3691, Cell Polarity, Migration and Cancer Unit, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France.
5
Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Département de la Recherche Fondamentale, Institut de biologie François Jacob, MIRCen, and CNRS UMR 9199, Université Paris-Sud, Neurodegenerative Diseases Laboratory, Fontenay-aux-Roses 92260, France.
6
Center for Interdisciplinary Research in Biology, Collège de France, CNRS UMR 7241, INSERM U1050, Labex Memolife, PSL Research University, Paris 75005, France nathalie.rouach@college-de-france.fr.

Abstract

Astrocytes undergo intense morphological maturation during development, changing from individual sparsely branched cells to polarized and tremendously ramified cells. Connexin 30, an astroglial gap-junction channel-forming protein expressed postnatally, regulates in situ the extension and ramification of astroglial processes. However, the involvement of connexin 30 in astroglial polarization, which is known to control cell morphology, remains unexplored. We found that connexin 30, independently of gap-junction-mediated intercellular biochemical coupling, alters the orientation of astrocyte protrusion, centrosome and Golgi apparatus during polarized migration in an in vitro wound-healing assay. Connexin 30 sets the orientation of astroglial motile protrusions via modulation of the laminin/β1 integrin/Cdc42 polarity pathway. Connexin 30 indeed reduces laminin levels, inhibits the redistribution of the β1-integrin extracellular matrix receptors, and inhibits the recruitment and activation of the small Rho GTPase Cdc42 at the leading edge of migrating astrocytes. In vivo, connexin 30, the expression of which is developmentally regulated, also contributes to the establishment of hippocampal astrocyte polarity during postnatal maturation. This study thus reveals that connexin 30 controls astroglial polarity during development.

KEYWORDS:

Astrocytes; Connexin; Development; Hippocampus; Mouse; Polarity

PMID:
29475972
PMCID:
PMC5869003
DOI:
10.1242/dev.155275
[Indexed for MEDLINE]
Free PMC Article

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