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Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2114-2119. doi: 10.1073/pnas.1525670115. Epub 2018 Feb 12.

Genetic resistance to purine nucleoside phosphorylase inhibition in Plasmodium falciparum.

Author information

1
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
2
Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
3
Department of Medicine, Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, NY 10461; johanna.daily@einstein.yu.edu vern.schramm@einstein.yu.edu.
4
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461.
5
Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461; johanna.daily@einstein.yu.edu vern.schramm@einstein.yu.edu.

Abstract

Plasmodium falciparum causes the most lethal form of human malaria and is a global health concern. The parasite responds to antimalarial therapies by developing drug resistance. The continuous development of new antimalarials with novel mechanisms of action is a priority for drug combination therapies. The use of transition-state analog inhibitors to block essential steps in purine salvage has been proposed as a new antimalarial approach. Mutations that reduce transition-state analog binding are also expected to reduce the essential catalytic function of the target. We have previously reported that inhibition of host and P. falciparum purine nucleoside phosphorylase (PfPNP) by DADMe-Immucillin-G (DADMe-ImmG) causes purine starvation and parasite death in vitro and in primate infection models. P. falciparum cultured under incremental DADMe-ImmG drug pressure initially exhibited increased PfPNP gene copy number and protein expression. At increased drug pressure, additional PfPNP gene copies appeared with point mutations at catalytic site residues involved in drug binding. Mutant PfPNPs from resistant clones demonstrated reduced affinity for DADMe-ImmG, but also reduced catalytic efficiency. The catalytic defects were partially overcome by gene amplification in the region expressing PfPNP. Crystal structures of native and mutated PfPNPs demonstrate altered catalytic site contacts to DADMe-ImmG. Both point mutations and gene amplification are required to overcome purine starvation induced by DADMe-ImmG. Resistance developed slowly, over 136 generations (2136 clonal selection). Transition-state analog inhibitors against PfPNP are slow to induce resistance and may have promise in malaria therapy.

KEYWORDS:

drug resistance; gene amplification; gene mutation; genetic resistance mechanisms; malaria

PMID:
29440412
PMCID:
PMC5834662
DOI:
10.1073/pnas.1525670115
[Indexed for MEDLINE]
Free PMC Article

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