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Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2090-2095. doi: 10.1073/pnas.1716161115. Epub 2018 Feb 9.

Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts.

Author information

1
Department of Animal Science, University of California, Davis, CA 95616.
2
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390; jun2.wu@utsouthwestern.edu belmonte@salk.edu pross@ucdavis.edu.
3
Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390.
4
Universidad Católica San Antonio de Murcia, 30107 Guadalupe, Murcia, Spain.
5
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037.
6
Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, 631-8505 Nara, Japan.
7
Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390.
8
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037; jun2.wu@utsouthwestern.edu belmonte@salk.edu pross@ucdavis.edu.
9
Department of Animal Science, University of California, Davis, CA 95616; jun2.wu@utsouthwestern.edu belmonte@salk.edu pross@ucdavis.edu.

Abstract

Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and biomedical perspectives, the derivation of stable ESCs from domestic ungulates is important for genomic testing and selection, genome engineering, and modeling human diseases. Cattle are one of the most important domestic ungulates that are commonly used for food and bioreactors. To date, however, it remains a challenge to produce stable pluripotent bovine ESC lines. Employing a culture system containing fibroblast growth factor 2 and an inhibitor of the canonical Wnt-signaling pathway, we derived pluripotent bovine ESCs (bESCs) with stable morphology, transcriptome, karyotype, population-doubling time, pluripotency marker gene expression, and epigenetic features. Under this condition bESC lines were efficiently derived (100% in optimal conditions), were established quickly (3-4 wk), and were simple to propagate (by trypsin treatment). When used as donors for nuclear transfer, bESCs produced normal blastocyst rates, thereby opening the possibility for genomic selection, genome editing, and production of cattle with high genetic value.

KEYWORDS:

bovine; embryonic stem cell; inner cell mass; pluripotency

Comment in

PMID:
29440377
PMCID:
PMC5834688
DOI:
10.1073/pnas.1716161115
[Indexed for MEDLINE]
Free PMC Article

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