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Development. 2018 Feb 8;145(3). pii: dev159707. doi: 10.1242/dev.159707.

GTL1 and DF1 regulate root hair growth through transcriptional repression of ROOT HAIR DEFECTIVE 6-LIKE 4 in Arabidopsis.

Author information

1
RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
2
Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27708, USA.
3
Biomathematics Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.
4
Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda 278-8510, Japan.
5
Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
6
Department of Biology, Howard Hughes Medical Institute, Duke University, Durham, NC 27695, USA.
7
RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan keiko.sugimoto@riken.jp.

Abstract

How plants determine the final size of growing cells is an important, yet unresolved, issue. Root hairs provide an excellent model system with which to study this as their final cell size is remarkably constant under constant environmental conditions. Previous studies have demonstrated that a basic helix-loop helix transcription factor ROOT HAIR DEFECTIVE 6-LIKE 4 (RSL4) promotes root hair growth, but how hair growth is terminated is not known. In this study, we demonstrate that a trihelix transcription factor GT-2-LIKE1 (GTL1) and its homolog DF1 repress root hair growth in Arabidopsis Our transcriptional data, combined with genome-wide chromatin-binding data, show that GTL1 and DF1 directly bind the RSL4 promoter and regulate its expression to repress root hair growth. Our data further show that GTL1 and RSL4 regulate each other, as well as a set of common downstream genes, many of which have previously been implicated in root hair growth. This study therefore uncovers a core regulatory module that fine-tunes the extent of root hair growth by the orchestrated actions of opposing transcription factors.

KEYWORDS:

Cell growth; Gene regulatory network; Root hair; Trihelix transcription factor

PMID:
29439132
PMCID:
PMC5818008
DOI:
10.1242/dev.159707
[Indexed for MEDLINE]
Free PMC Article

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