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Mol Biotechnol. 2018 Mar;60(3):203-214. doi: 10.1007/s12033-018-0063-x.

Xerophyta viscosa Aldose Reductase, XvAld1, Enhances Drought Tolerance in Transgenic Sweetpotato.

Author information

1
Department of Biochemistry and Biotechnology, Pwani University, P.O Box 195-80108, Kilifi, Kenya. wilton.mbinda@gmail.com.
2
Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya. wilton.mbinda@gmail.com.
3
Department of Plant Sciences, Kenyatta University, Nairobi, Kenya.
4
Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
5
Department of Biochemistry and Biotechnology, Kenyatta University, Nairobi, Kenya.

Abstract

Sweetpotato is a significant crop which is widely cultivated particularly in the developing countries with high and stable yield. However, drought stress is a major limiting factor that antagonistically influences the crop's productivity. Dehydration stress caused by drought causes aggregation of reactive oxygen species (ROS) in plants, and aldose reductases are first-line safeguards against ROS caused by oxidative stress. In the present study, we generated transgenic sweetpotato plants expressing aldose reductase, XvAld1 isolated from Xerophyta viscosa under the control of a stress-inducible promoter via Agrobacterium-mediated transformation. Our results demonstrated that the transgenic sweetpotato lines displayed significant enhanced tolerance to simulated drought stress and enhanced recuperation after rehydration contrasted with wild-type plants. In addition, the transgenic plants exhibited improved photosynthetic efficiency, higher water content and more proline accumulation under dehydration stress conditions compared with wild-type plants. These results demonstrate that exploiting the XvAld1 gene is not only a compelling and attainable way to improve sweetpotato tolerance to drought stresses without causing any phenotypic imperfections but also a promising gene candidate for more extensive crop improvement.

KEYWORDS:

Drought stress; Genetic transformation; Ipomoea batatas; Reactive oxygen species; Sweetpotato; Xerophyta viscosa

PMID:
29423655
DOI:
10.1007/s12033-018-0063-x
[Indexed for MEDLINE]

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