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Adv Mater. 2018 Mar;30(12):e1706785. doi: 10.1002/adma.201706785. Epub 2018 Jan 24.

Oriented Nanofibrous Polymer Scaffolds Containing Protein-Loaded Porous Silicon Generated by Spray Nebulization.

Author information

1
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
2
School of Pharmacy, University of Queensland, 20 Cornwall Street, Woolloongabba, Brisbane, Queensland, 4102, Australia.
3
Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul, 02447, Republic of Korea.
4
Department of Nanoengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
5
Materials Science and Engineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
6
Molecular Neurobiology Laboratory, Salk Institute for Biological Studies, 10010 N Torrey Pines, La Jolla, CA, 92037, USA.
7
Veterans Administration Medical Center, 3350 La Jolla Village Drive, San Diego, CA, 92161, USA.
8
Department of Neurosciences, University of California, San Diego, 9500, Gilman, La Jolla, CA, 92093, USA.

Abstract

Oriented composite nanofibers consisting of porous silicon nanoparticles (pSiNPs) embedded in a polycaprolactone or poly(lactide-co-glycolide) matrix are prepared by spray nebulization from chloroform solutions using an airbrush. The nanofibers can be oriented by an appropriate positioning of the airbrush nozzle, and they can direct growth of neurites from rat dorsal root ganglion neurons. When loaded with the model protein lysozyme, the pSiNPs allow the generation of nanofiber scaffolds that carry and deliver the protein under physiologic conditions (phosphate-buffered saline (PBS), at 37 °C) for up to 60 d, retaining 75% of the enzymatic activity over this time period. The mass loading of protein in the pSiNPs is 36%, and in the resulting polymer/pSiNP scaffolds it is 3.6%. The use of pSiNPs that display intrinsic photoluminescence (from the quantum-confined Si nanostructure) allows the polymer/pSiNP composites to be definitively identified and tracked by time-gated photoluminescence imaging. The remarkable ability of the pSiNPs to protect the protein payload from denaturation, both during processing and for the duration of the long-term aqueous release study, establishes a model for the generation of biodegradable nanofiber scaffolds that can load and deliver sensitive biologics.

KEYWORDS:

airbrush; cell guidance; controlled release drug delivery; polycaprolactone; protein therapeutics; time-gated photoluminescence imaging; tissue engineering

PMID:
29363828
DOI:
10.1002/adma.201706785

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