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Mol Microbiol. 2018 Apr;108(1):16-31. doi: 10.1111/mmi.13912. Epub 2018 Feb 12.

Dynamical localization of a thylakoid membrane binding protein is required for acquisition of photosynthetic competency.

Gutu A1,2,3, Chang F2, O'Shea EK1,2,3.

Author information

1
Howard Hughes Medical Institute, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Cambridge, MA 02138, USA.
2
Department of Molecular and Cellular Biology, Harvard University Faculty of Arts and Sciences, Cambridge, MA 02138, USA.
3
Department of Chemistry and Chemical Biology, Harvard University Faculty of Arts and Sciences Center for Systems Biology, Cambridge, MA 02138, USA.

Abstract

Vipp1 is highly conserved and essential for photosynthesis, but its function is unclear as it does not participate directly in light-dependent reactions. We analyzed Vipp1 localization in live cyanobacterial cells and show that Vipp1 is highly dynamic, continuously exchanging between a diffuse fraction that is uniformly distributed throughout the cell and a punctate fraction that is concentrated at high curvature regions of the thylakoid located at the cell periphery. Experimentally perturbing the spatial distribution of Vipp1 by relocalizing it to the nucleoid causes a severe growth defect during the transition from non-photosynthetic (dark) to photosynthetic (light) growth. However, the same perturbation of Vipp1 in dark alone or light alone growth conditions causes no growth or thylakoid morphology defects. We propose that the punctuated dynamics of Vipp1 at the cell periphery in regions of high thylakoid curvature enable acquisition of photosynthetic competency, perhaps by facilitating biogenesis of photosynthetic complexes involved in light-dependent reactions of photosynthesis.

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PMID:
29357135
PMCID:
PMC5910887
[Available on 2019-04-01]
DOI:
10.1111/mmi.13912

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