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Anal Chim Acta. 2018 Feb 13;1000:223-231. doi: 10.1016/j.aca.2017.11.050. Epub 2017 Nov 25.

Separation of pathogenic bacteria by chain length.

Author information

1
Division of Solid State Physics and NanoLund, Lund University, SE-221 00, Sweden.
2
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
3
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, SE-171 77 Stockholm, Sweden; Department of Clinical Microbiology, Karolinska University Hospital, SE-171 76 Stockholm, Sweden; SCELSE and LKC, Nanyang Technological University, NTU, Singapore 639798, Singapore.
4
Division of Solid State Physics and NanoLund, Lund University, SE-221 00, Sweden. Electronic address: jonas.tegenfeldt@ftf.lth.se.

Abstract

Using Deterministic Lateral Displacement devices optimized for sensitivity to particle length, we separate subpopulations of bacteria depending on known properties that affect their capability to cause disease (virulence). For the human bacterial pathogen Streptococcus pneumoniae, bacterial chain length and the presence of a capsule are known virulence factors contributing to its ability to cause severe disease. Separation of cultured pneumococci into subpopulations based on morphological type (single cocci, diplococci and chains) will enable more detailed studies of the role they play in virulence. Moreover, we present separation of mixed populations of almost genetically identical encapsulated and non-encapsulated pneumococcal strains in our device.

KEYWORDS:

Bacterial virulence; Deterministic lateral displacement; Morphology; Separation

PMID:
29289314
DOI:
10.1016/j.aca.2017.11.050
[Indexed for MEDLINE]
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