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Neuroimage. 2018 Apr 1;169:374-382. doi: 10.1016/j.neuroimage.2017.12.054. Epub 2017 Dec 19.

Mn2+ dynamics in manganese-enhanced MRI (MEMRI): Cav1.2 channel-mediated uptake and preferential accumulation in projection terminals.

Author information

1
Max Planck Institute of Psychiatry, Dept. Stress Neurobiology & Neurogenetics, Kraepelinstr. 2-10, 80804 Munich, Germany; Max Planck Institute of Psychiatry, Core Unit Neuroimaging, Kraepelinstr. 2-10, 80804 Munich, Germany.
2
Max Planck Institute of Psychiatry, Dept. Stress Neurobiology & Neurogenetics, Kraepelinstr. 2-10, 80804 Munich, Germany.
3
Max Planck Institute of Psychiatry, Core Unit Neuroimaging, Kraepelinstr. 2-10, 80804 Munich, Germany.
4
Max Planck Institute of Psychiatry, Dept. Stress Neurobiology & Neurogenetics, Kraepelinstr. 2-10, 80804 Munich, Germany. Electronic address: wotjak@psych.mpg.de.

Abstract

Manganese-enhanced magnetic resonance imaging (MEMRI) exploits the biophysical similarity of Ca2+ and Mn2+ to map the brain's activity in vivo. However, to what extent different Ca2+ channels contribute to the enhanced signal that MEMRI provides and how Mn2+ dynamics influence Mn2+ brain accumulation after systemic administration of MnCl2 are not yet fully understood. Here, we demonstrate that mice lacking the L-type Ca2+ channel 1.2 (Cav1.2) in the CNS show approximately 50% less increase in MEMRI contrast after repeated systemic MnCl2 injections, as compared to control mice. In contrast, genetic deletion of L-type Ca2+ channel 1.3 (Cav1.3) did not reduce signal. Brain structure- or cell type-specific deletion of Cav1.2 in combination with voxel-wise MEMRI analysis revealed a preferential accumulation of Mn2+ in projection terminals, which was confirmed by local MnCl2 administration to defined brain areas. Taken together, we provide unequivocal evidence that Cav1.2 represents an important channel for neuronal Mn2+ influx after systemic injections. We also show that after neuronal uptake, Mn2+ preferentially accumulates in projection terminals.

KEYWORDS:

Activity-dependent; CACNA1C; Calcium channels; Connectomics; Functional imaging; Manganese enhanced MRI

[Indexed for MEDLINE]

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