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Biomed Pharmacother. 2017 Dec;96:1216-1221. doi: 10.1016/j.biopha.2017.11.096. Epub 2017 Nov 26.

LncRNA HOXD-AS1 promotes epithelial ovarian cancer cells proliferation and invasion by targeting miR-133a-3p and activating Wnt/β-catenin signaling pathway.

Author information

1
Department of Gynecology and Oncology I, Xinxiang Central Hospital, Xinxiang 453000 Henan China. Electronic address: doc_zhangying007@sohu.com.
2
Institute of Biomedical Engineering, Xinxiang Medical University, Xinxiang 453000, Henan China.
3
Department of Pathology, Zhoukou Central Hospital, Zhoukou 466000, Henan China.
4
Department of Obstetrics and Gynecology, the Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China.

Abstract

BACKGROUND:

Long non-coding RNA (lncRNA) HOXD cluster antisense RNA 1 (HOXD-AS1) functions as a crucial regulator in the progression and development of tumors. The aim of this study is to unravel the underlying mechanisms of HOXD-AS1 on epithelial ovarian cancer (EOC).

METHODS:

43 paired EOC tissues and adjacent non-tumor tissues were collected postoperatively from patients. QRT-PCR was used to explore HOXD-AS1 expression in both EOC tissues and cell lines. Cell proliferation and invasion were monitored by MTT assay and transwell invasion assay.

RESULTS:

In the current study, we found that the expression of HOXD-AS1 was upregulated in EOC tissues and cell lines. High HOXD-AS1 expression was correlated with advanced FIGO stage, lymph node metastasis, and poor overall survival in EOC patients. We also showed that HOXD-AS1 promoted cell proliferation, invasion, and epithelial-mesenchymal transition (EMT) via activating Wnt/β-catenin signaling in EOC cells. Furthermore, we found that miR-133a-3p was a direct downstream target of HOXD-AS1 in EOC. HOXD-AS1 promoted cell proliferation, invasion, and EMT process through sponging miR-133a-3p in EOC cells.

CONCLUSION:

Our study indicated that lncRNA HOXD-AS1 promoted the proliferation, invasion, and EMT process of EOC cells via targeting miR-133a-3p and activating Wnt/β-catenin signaling pathway.

KEYWORDS:

HOXD-AS1; Wnt/β-catenin; lncRNA; miR-133a-3p

PMID:
29239819
DOI:
10.1016/j.biopha.2017.11.096
[Indexed for MEDLINE]

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