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Stem Cell Reports. 2017 Dec 12;9(6):2018-2033. doi: 10.1016/j.stemcr.2017.10.027. Epub 2017 Nov 30.

Coupling between Myogenesis and Angiogenesis during Skeletal Muscle Regeneration Is Stimulated by Restorative Macrophages.

Author information

1
Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, PRES Sorbonne-Paris-Cité, 75014 Paris, France; Institut Pasteur, Infection and Epidemiology Department, Histopathology and Animal Models Unit, 75015 Paris, France.
2
Institut NeuroMyoGène, Université Lyon, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5534, 16 rue Raphael Dubois, Bâtiment Gregor Mendel, 69100 Villeurbanne, France.
3
Collège de France, Center of Interdisciplinary Research in Biology, CNRS UMR7241, INSERM U1050, 75005 Paris, France.
4
Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, PRES Sorbonne-Paris-Cité, 75014 Paris, France.
5
Institut Pasteur, Infection and Epidemiology Department, Histopathology and Animal Models Unit, 75015 Paris, France.
6
Institut Cochin, INSERM U1016, CNRS UMR8104, Université Paris Descartes, PRES Sorbonne-Paris-Cité, 75014 Paris, France; Institut NeuroMyoGène, Université Lyon, Université Claude Bernard Lyon 1, INSERM U1217, CNRS UMR5534, 16 rue Raphael Dubois, Bâtiment Gregor Mendel, 69100 Villeurbanne, France. Electronic address: benedicte.chazaud@inserm.fr.

Abstract

In skeletal muscle, new functions for vessels have recently emerged beyond oxygen and nutrient supply, through the interactions that vascular cells establish with muscle stem cells. Here, we demonstrate in human and mouse that endothelial cells (ECs) and myogenic progenitor cells (MPCs) interacted together to couple myogenesis and angiogenesis in vitro and in vivo during skeletal muscle regeneration. Kinetics of gene expression of ECs and MPCs sorted at different time points of regeneration identified three effectors secreted by both ECs and MPCs. Apelin, Oncostatin M, and Periostin were shown to control myogenesis/angiogenesis coupling in vitro and to be required for myogenesis and vessel formation during muscle regeneration in vivo. Furthermore, restorative macrophages, which have been previously shown to support myogenesis in vivo, were shown in a 3D triculture model to stimulate myogenesis/angiogenesis coupling, notably through Oncostatin M production. Our data demonstrate that restorative macrophages orchestrate muscle regeneration by controlling myogenesis/angiogenesis coupling.

KEYWORDS:

angiogenesis; macrophages; muscle stem cells; myogenesis; skeletal muscle regeneration

PMID:
29198825
PMCID:
PMC5785732
DOI:
10.1016/j.stemcr.2017.10.027
[Indexed for MEDLINE]
Free PMC Article

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