Format

Send to

Choose Destination
Cold Spring Harb Protoc. 2017 Nov 1;2017(11):pdb.prot099366. doi: 10.1101/pdb.prot099366.

In Vivo Transfection of Naked DNA into Xenopus Tadpole Tail Muscle.

Author information

1
Evolution des Régulations Endocriniennes, Département RDDM, CNRS UMR 7221, MNHN, Sorbonne Université, Paris, France.
2
Evolution des Régulations Endocriniennes, Département RDDM, CNRS UMR 7221, MNHN, Sorbonne Université, Paris, France laurent.coen@mnhn.fr.

Abstract

In vivo gene transfer systems are important to study foreign gene expression and promoter regulation in an organism, with the benefit of exploring this in an integrated environment. Direct injection of plasmids encoding exogenous promoters and genes into muscle has numerous advantages: the protocol is easy, efficient, and shows time-persistent plasmid expression in transfected muscular cells. After injecting naked-DNA plasmids into tadpole tail muscle, transgene expression is strong, reproducible, and correlates with the amount of DNA injected. Moreover, expression is stable as long as the tadpoles remain, or are maintained, in premetamorphic stages. By directly expressing genes and regulated promoters in Xenopus tadpole muscle in vivo, one can exploit the powerful experimental advantages of gene transfer systems in an intact, physiologically normal animal.

PMID:
29093206
DOI:
10.1101/pdb.prot099366
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center