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Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9989-E9998. doi: 10.1073/pnas.1710964114. Epub 2017 Oct 31.

Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq.

Author information

1
Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
2
Nephrology Clinic, National Cancer Center, Goyang, 10408, South Korea.
3
Center for Systems Biology, Program in Membrane Biology and Division of Nephrology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114.
4
Division of Nephrology, Hypertension and Renal Transplantation, University of Florida College of Medicine, Gainesville, FL 32610.
5
Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322.
6
Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322.
7
Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892; burgm@nhlbi.nih.gov knepperm@nhlbi.nih.gov.

Abstract

Prior RNA sequencing (RNA-seq) studies have identified complete transcriptomes for most renal epithelial cell types. The exceptions are the cell types that make up the renal collecting duct, namely intercalated cells (ICs) and principal cells (PCs), which account for only a small fraction of the kidney mass, but play critical physiological roles in the regulation of blood pressure, extracellular fluid volume, and extracellular fluid composition. To enrich these cell types, we used FACS that employed well-established lectin cell surface markers for PCs and type B ICs, as well as a newly identified cell surface marker for type A ICs, c-Kit. Single-cell RNA-seq using the IC- and PC-enriched populations as input enabled identification of complete transcriptomes of A-ICs, B-ICs, and PCs. The data were used to create a freely accessible online gene-expression database for collecting duct cells. This database allowed identification of genes that are selectively expressed in each cell type, including cell-surface receptors, transcription factors, transporters, and secreted proteins. The analysis also identified a small fraction of hybrid cells expressing aquaporin-2 and anion exchanger 1 or pendrin transcripts. In many cases, mRNAs for receptors and their ligands were identified in different cells (e.g., Notch2 chiefly in PCs vs. Jag1 chiefly in ICs), suggesting signaling cross-talk among the three cell types. The identified patterns of gene expression among the three types of collecting duct cells provide a foundation for understanding physiological regulation and pathophysiology in the renal collecting duct.

KEYWORDS:

intercalated cell; kidney; principal cell; systems biology

PMID:
29089413
PMCID:
PMC5699061
DOI:
10.1073/pnas.1710964114
[Indexed for MEDLINE]
Free PMC Article

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