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Clin Microbiol Infect. 2018 Jun;24(6):660.e1-660.e4. doi: 10.1016/j.cmi.2017.10.009. Epub 2017 Oct 21.

Predominance of G9P[8] rotavirus strains throughout France, 2014-2017.

Author information

1
National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, Université de Bourgogne Franche-Comté/AgroSup Dijon, France; French Rotavirus Network, France. Electronic address: jerome.kaplon@chu-dijon.fr.
2
National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; French Rotavirus Network, France.
3
French Rotavirus Network, France; Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, France.
4
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Brest, France.
5
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Caen, France.
6
French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Cahors, France.
7
French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Charleville-Mézières, France.
8
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Lille, France.
9
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Limoges, France.
10
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Lyon, France.
11
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Montpellier, France.
12
French Rotavirus Network, France; Laboratory of Clinical Biology, Hospital of Orléans, France.
13
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Paris, France.
14
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Poitiers, France.
15
French Rotavirus Network, France; Laboratory of Clinical Virology, University Hospital of Rennes, France.
16
National Reference Centre for Gastroenteritis Viruses, Laboratory of Biology and Pathology, University Hospital of Dijon, France; UMR PAM A 02.102 Procédés Alimentaires et Microbiologiques, Université de Bourgogne Franche-Comté/AgroSup Dijon, France; French Rotavirus Network, France. Electronic address: alexis.de-rougemont@u-bourgogne.fr.

Abstract

OBJECTIVES:

Group A rotavirus is a major cause of acute gastroenteritis in young children worldwide. A prospective surveillance network has been set up in France to investigate rotavirus infections and to detect the emergence of potentially epidemic strains.

METHODS:

From 2014 to 2017, rotavirus-positive stool samples were collected from 2394 children under 5 years old attending the paediatric emergency units of 13 large hospitals. Rotaviruses were genotyped by RT-PCR with regard to their outer capsid proteins VP4 and VP7.

RESULTS:

Genotyping of 2421 rotaviruses showed that after a marked increase in G9P[8] (32.1%) during the 2014-2015 season, G9P[8] became the predominant genotype during the 2015-2016 and 2016-2017 seasons with detection rates of 64.1% and 77.3%, respectively, whereas G1P[8] were detected at low rates of 16.8% and 6.6%, respectively. Phylogenetic analysis of the partial rotavirus VP7 and VP4 coding genes revealed that all of these G9P [8] strains belonged to the lineage III and the P [8]-3 lineage, respectively, and shared the same genetic background (G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) as did most of previously detected G9P[8] strains and particularly the emerging G9P[8] strains from the 2004-2005 season in France.

CONCLUSIONS:

G9P[8] rotaviruses have become the predominant circulating genotype for the first time since their emergence a decade ago. In the absence of rotavirus immunization programmes in France, our data give an insight into the natural fluctuation of rotavirus genotypes in a non-vaccinated population and provide a base line for a better interpretation of data in European countries with routine rotavirus vaccination.

KEYWORDS:

G9P[8]; gastroenteritis; genotype; rotavirus; vaccine

PMID:
29066401
DOI:
10.1016/j.cmi.2017.10.009
[Indexed for MEDLINE]
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