Format

Send to

Choose Destination
Mol Cell Biol. 1988 Apr;8(4):1525-33.

Double minute chromosomes can be produced from precursors derived from a chromosomal deletion.

Author information

1
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, California 92037.

Abstract

Recent experiments have shown that gene amplification can be mediated by submicroscopic, autonomously replicating, circular extrachromosomal molecules. We refer to those molecules as episomes (S. Carroll, P. Gaudray, M. L. DeRose, J. F. Emery, J. L. Meinkoth, E. Nakkim, M. Subler, D. D. Von Hoff, and G. M. Wahl, Mol. Cell. Biol. 7:1740-1750, 1987). The experiments reported in this paper explore the way episomes are formed and their fate in the cell over time. The data reveal that in our system the episomes are initially 250 kilobases, but gradually enlarge until they become double minute chromosomes. In addition, we show that episomes or double minute chromosomes can integrate into chromosomes. Our results also suggest that episomes can be produced by deletion of the corresponding sequences from the chromosome.

PMID:
2898098
PMCID:
PMC363312
DOI:
10.1128/mcb.8.4.1525
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center