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J Infect Dis. 2017 Sep 15;216(6):736-743. doi: 10.1093/infdis/jix394.

Insights Into Onchocerca volvulus Population Biology Through Multilocus Immunophenotyping.

Author information

1
Laboratory of Parasitic Diseases.
2
Laboratory of Malaria and Vector Research.
3
Biostatistics Research Branch, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
4
McDonnell Genome Institute.
5
Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, St Louis, Missouri.

Abstract

We have developed a serologically based immunophenotyping approach to study Onchocerca volvulus (Ov) population diversity. Using genomic sequence data and polymerase chain reaction-based genotyping, we identified nonsynonymous single-nucleotide polymorphisms (SNPs) in the genes of 16 major immunogenic Ov proteins: Ov-CHI-1/Ov-CHI-2, Ov16, Ov-FAR-1, Ov-CPI-1, Ov-B20, Ov-ASP-1, Ov-TMY-1, OvSOD1, OvGST1, Ov-CAL-1, M3/M4, Ov-RAL-1, Ov-RAL-2, Ov-ALT-1, Ov-FBA-1, and Ov-B8. We assessed the immunoreactivity of onchocerciasis patient sera (n = 152) from the Americas, West Africa, Central Africa, and East Africa against peptides derived from 10 of these proteins containing SNPs. Statistically significant variation in immunoreactivity among the regions was seen in SNP-containing peptides derived from 8 of 10 proteins tested: OVOC1192(1-15), OVOC9988(28-42), OVOC9225(320-334), OVOC7453(22-36), OVOC11517(14-28), OVOC3177(283-297), OVOC7911(594-608), and OVOC12628(174-188). Our data show that differences in immunoreactivity to variant antigenic peptides may be used to characterize Ov populations, thereby elucidating features of Ov population biology previously inaccessible because of the limited availability of parasite material.

KEYWORDS:

Onchocerca volvulus; immunophenotype; immunoreactivity; population biology; single nucleotide polymorphism

PMID:
28934436
PMCID:
PMC5853885
DOI:
10.1093/infdis/jix394
[Indexed for MEDLINE]
Free PMC Article

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