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J Leukoc Biol. 2018 Feb;103(2):321-339. doi: 10.1189/jlb.5MA0517-207R. Epub 2017 Dec 29.

Perturbed CD8+ T cell immunity across universal influenza epitopes in the elderly.

Author information

1
Department of Microbiology and Immunology, The University of Melbourne, Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia.
2
Division of Infection and Immunity, Cardiff University School of Medicine, Heath Park, Cardiff, United Kingdom.
3
Infection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
4
Pasteur School of Public Health, The University of Hong Kong, Hong Kong SAR, Republic of China.
5
Australian Research Council Centre of Excellence for Advanced Molecular Imaging, Monash University, Clayton, Victoria, Australia.
6
Obstetrics, Nutrition and Endocrinology Group, Department of Obstetrics and Gynaecology, The University of Melbourne, Victoria, Australia.
7
Mercy Perinatal Research Centre, Mercy Hospital for Women, Heidelberg, Victoria, Australia.
8
Department of Infectious Diseases, Alfred Hospital and Monash University, Melbourne, Victoria, Australia.
9
Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia.
10
Deepdene Surgery, Deepdene, Victoria, Australia.

Abstract

Influenza epidemics lead to severe illness, life-threatening complications, and deaths, especially in the elderly. As CD8+ T cells are associated with rapid recovery from influenza, we investigated the effects of aging on antigen-specific CD8+ T cells across the universal influenza epitopes in humans. We show that aging is characterized by altered frequencies in T cell subsets, with naive T cells being partially replaced by activated effector/memory populations. Although we observed no striking differences in TCR signaling capacity, T cells in the elderly had increased expression of transcription factors Eomes and T-bet, and such changes were most apparent in CD8+ T cells. Strikingly, the numbers of antigen-specific CD8+ T cells across universal influenza epitopes were reduced in the elderly, although their effector/memory phenotypes remained stable. To understand whether diminished numbers of influenza-specific CD8+ T cells in the elderly resulted from alteration in TCR clonotypes, we dissected the TCRαβ repertoire specific for the prominent HLA-A*02:01-restricted-M158-66 (A2/M158 ) influenza epitope. We provide the first ex vivo data on paired antigen-specific TCRαβ clonotypes in the elderly, showing that influenza-specific A2/M158+ TCRαβ repertoires in the elderly adults varied from those in younger adults, with the main features being a reduction in the frequency of the public TRAV27-TRBV19 TCRαβ clonotype, increased proportion of private TCRαβ signatures, broader use of TRAV and TRBV gene segments, and large clonal expansion of private TCRαβ clonotypes with longer CDR3 loops. Our study supports the development of T cell-targeted influenza vaccines that would boost the T cell compartment during life and maintain the numbers and optimal TCRαβ signatures in the elderly.

KEYWORDS:

CD8+ T cells; TCR repertoire; ZAP-70; aging; transcription factors

PMID:
28928269
DOI:
10.1189/jlb.5MA0517-207R

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