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Sci Rep. 2017 Sep 5;7(1):10487. doi: 10.1038/s41598-017-08596-5.

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs.

Author information

1
Salk Institute for Biological Studies, 10010 N. Torrey Pines Rd., La Jolla, CA, 92037, USA.
2
Universidad Católica San Antonio de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107, Spain.
3
Department of Animal Science, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA.
4
Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, 3327-204 Nakamachi, Nara, 631-8505, Japan.
5
School of Veterinary Medicine, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA.
6
Department of Animal Science, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA. pross@ucdavis.edu.
7
Salk Institute for Biological Studies, 10010 N. Torrey Pines Rd., La Jolla, CA, 92037, USA. belmonte@salk.edu.

Abstract

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

PMID:
28874671
PMCID:
PMC5585254
DOI:
10.1038/s41598-017-08596-5
[Indexed for MEDLINE]
Free PMC Article

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