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J Immunol. 2017 Oct 1;199(7):2451-2459. doi: 10.4049/jimmunol.1700571. Epub 2017 Aug 30.

Chemokine Receptor-Dependent Control of Skin Tissue-Resident Memory T Cell Formation.

Author information

1
Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000 Australia.
2
Australian Research Council Centre of Excellence in Advanced Molecular Imaging, The University of Melbourne, Melbourne, Victoria 3000, Australia.
3
Division of Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia.
4
Division of Molecular Immunology, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia; and.
5
Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3010, Australia.
6
Department of Microbiology and Immunology, The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria 3000 Australia; smue@unimelb.edu.au.

Abstract

Infection or inflammation of the skin recruits effector CD8+ T cells that enter the epidermis and form populations of long-lived tissue-resident memory T (TRM) cells. These skin TRM cells migrate within the constrained epidermal environment by extending multiple dynamic dendritic projections and squeezing between keratinocytes to survey the tissue for pathogens. In this study, we examined the signals required for this distinctive mode of T cell migration by inhibiting key cytoskeletal components and performing intravital two-photon microscopy to visualize TRM cell behavior. We found that TRM cell motility and dendrite formation required an intact actomyosin cytoskeleton and the Rho-associated coiled-coil containing kinases. We also identified an essential role for microtubules for maintaining skin TRM cell shape and cellular integrity. We reveal a role for pertussis toxin-sensitive signaling for TRM cell dendritic morphology and migration that is independent of CXCR3 or CXCR6, or the skin-selective chemokine receptors CCR10 and CCR8. However, we found that CXCR6 and CCR10 expression by CD8+ T cells was required for the optimal formation of memory T cell populations, in particular TRM cell populations in the skin.

PMID:
28855310
DOI:
10.4049/jimmunol.1700571
[Indexed for MEDLINE]
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