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Arch Virol. 2017 Dec;162(12):3619-3627. doi: 10.1007/s00705-017-3519-6. Epub 2017 Aug 16.

Genomic characterization of a novel calicivirus, FHMCV-2012, from baitfish in the USA.

Author information

1
Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA. kumars@umn.edu.
2
Minnesota Aquatic Invasive Species Research Center, Department of Fisheries, Wildlife, and Conservation Biology, College of Food, Agriculture, and Natural Resource Sciences, University of Minnesota, St. Paul, MN, 55108, USA.
3
College of Veterinary Medicine, University of Georgia, 501 D. W. Brooks Drive, Athens, GA, 30602, USA.
4
Department of Infectious Diseases and Pathology, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
5
Minnesota Veterinary Diagnostic Laboratory, Department of Veterinary Population Medicine, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN, 55108, USA.
6
US Fish and Wildlife Service, La Crosse Fish Health Center, 555 Lester Avenue, Onalaska, WI, 54650, USA.

Abstract

During regulatory sampling of fathead minnows (Pimephales promelas), a novel calicivirus was isolated from homogenates of kidney and spleen inoculated into bluegill fry (BF-2) cells. Infected cell cultures exhibiting cytopathic effects were screened by PCR-based methods for selected fish viral pathogens. Illumina HiSeq next generation sequencing of the total RNA revealed a novel calicivirus genome that showed limited protein sequence similarity to known homologs in a BLASTp search. The complete genome of this fathead minnow calicivirus (FHMCV) is 6564 nt long, encoding a polyprotein of 2114 aa in length. The complete polyprotein shared only 21% identity with Atlantic salmon calicivirus,followed by 11% to 14% identity with mammalian caliciviruses. A molecular detection assay (RT-PCR) was designed from this sequence for screening of field samples for FHMCV in the future. This virus likely represents a prototype species of a novel genus in the family Caliciviridae, tentatively named "Minovirus".

PMID:
28815386
DOI:
10.1007/s00705-017-3519-6
[Indexed for MEDLINE]

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