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Arch Microbiol. 2017 Dec;199(10):1383-1389. doi: 10.1007/s00203-017-1412-z. Epub 2017 Jul 24.

Detection of Verticillium species in Swedish soils using real-time PCR.

Author information

1
Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007, Uppsala, Sweden. Georgios.Tzelepis@slu.se.
2
Department of Plant Biology, Uppsala BioCenter, Linnean Center for Plant Biology, Swedish University of Agricultural Sciences, P.O. Box 7080, 75007, Uppsala, Sweden.
3
Department of Plant Virology, Institute of Agricultural Sciences (IAGS), University of the Punjab, Quaid-e-Azam Campus, Box. 54590, Lahore, Pakistan.
4
National Food Agency, Sweden, Box 622, 751 26, Uppsala, Sweden.

Abstract

Verticillium species are soilborne plant pathogens, responsible for big yield losses worldwide. Here, we report improved procedures to generate DNA from Verticillium species imbedded in farm soils. Using new genomic sequence information, primers for V. dahliae, V. albo-atrum, V. tricorpus, and V. longisporum were designed. In a survey of 429 samples from intensively farmed soil of two Swedish regions, only V. dahliae and V. longisporum were identified. A bias towards V. longisporum (40%) was seen in the south, whereas V. dahliae was more frequent in the western region (19%). Analyses of soil and leaf samples from 20 sugar beet fields, where foliar wilting had been observed, revealed V. dahliae DNA in all leaf and soil samples and V. longisporum in 18 soil samples, illustrating host choice and longevity of the V. longisporum microsclerotia. This study demonstrates the applicability of new molecular diagnostic tools that are important for growers of variable crops.

KEYWORDS:

Beta vulgaris; Brassica napus; Soilborne pathogens; Verticillium; qPCR

PMID:
28741076
PMCID:
PMC5663805
DOI:
10.1007/s00203-017-1412-z
[Indexed for MEDLINE]
Free PMC Article

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