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Anal Chem. 2017 Aug 15;89(16):8496-8500. doi: 10.1021/acs.analchem.7b02059. Epub 2017 Jul 24.

Highly Selective and Sensitive Two-Photon Fluorescence Probe for Endogenous Peroxynitrite Detection and Its Applications in Living Cells and Tissues.

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Department of Chemistry and Nano Science, Ewha Womans University , Seoul 120-750, Korea.
Department of Chemistry and Energy Systems Research, Ajou University , Suwon 443-749, Korea.


A new two-photon fluorescence probe for endogenous peroxynitrite (ONOO-) detection was designed and synthesized. The probe exhibits good selectivity and sensitivity for ONOO- in phosphate-buffered saline solution with a low detection limit (3.5 × 10-8 M). Furthermore, the probe displays good performance in detecting endogenous ONOO-, not only in RAW 264.7 cells but also in rat hippocampal tissue, with a high two-photon cross-section value (δ ≈ 100 GM) at a deep depth of 120 μm.

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