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Comp Biochem Physiol C Toxicol Pharmacol. 2017 Oct;200:52-58. doi: 10.1016/j.cbpc.2017.06.006. Epub 2017 Jun 17.

Using short-term bioassays to evaluate the endocrine disrupting capacity of the pesticides linuron and fenoxycarb.

Author information

1
Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France; UMR CNRS 5166, Evolution des Régulations Endocriniennes, Department of Regulation, Development and Molecular Diversity, Muséum National d'Histoire Naturelle, Paris, France.
2
Department of Physiology, Anatomy and Microbiology, La Trobe University, Bundoora, Victoria 3086, Australia.
3
Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France.
4
UMR CNRS 5166, Evolution des Régulations Endocriniennes, Department of Regulation, Development and Molecular Diversity, Muséum National d'Histoire Naturelle, Paris, France.
5
Laboratoire WatchFrog S.A., 1 Rue Pierre Fontaine, 91000 Évry, France. Electronic address: pasquier@watchfrog.fr.

Abstract

Several short-term whole-organism bioassays based on transgenic aquatic models are now under validation by the OECD (Organization for Economic Co-operation and Development) to become standardized test guidelines for the evaluation of the endocrine activity of substances. Evaluation of the endocrine disrupting capacity of pesticides will be a domain of applicability of these future reference tests. The herbicide linuron and the insecticide fenoxycarb are two chemicals commonly used in agricultural practices. While numerous studies indicate that linuron is likely to be an endocrine disruptor, there is little information available on the effect of fenoxycarb on vertebrate endocrine systems. Using whole-organism bioassays based on transgenic Xenopus laevis tadpoles and medaka fry we assessed the potential of fenoxycarb and linuron to disrupt thyroid, androgen and estrogen signaling. In addition we used in silico approach to simulate the affinity of these two pesticides to human hormone receptors. Linuron elicited thyroid hormone-like activity in tadpoles at all concentrations tested and, showed an anti-estrogenic activity in medaka at concentrations 2.5mg/L and higher. Our experiments suggest that, in addition to its previously established anti-androgenic action, linuron exhibits thyroid hormone-like responses, as well as acting at the estrogen receptor level to inhibit estrogen signaling. Fenoxycarb on the other hand, did not cause any changes in thyroid, androgen or estrogen signaling at the concentrations tested.

KEYWORDS:

Androgen; Endocrine disruptors; Estrogen; Fenoxycarb; Linuron; Thyroid, XETA, RADAR

PMID:
28634053
DOI:
10.1016/j.cbpc.2017.06.006
[Indexed for MEDLINE]

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