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Microb Pathog. 2017 Aug;109:305-312. doi: 10.1016/j.micpath.2017.06.001. Epub 2017 Jun 20.

Detection of Aichi virus genotype B in two lines of wastewater treatment processes.

Author information

1
Centre of Research and Water Technologies (CERTE), Laboratory of Treatment and Wastewater Valorisation, 8020, Techno Park of Borj-Cedria, Tunisia; Faculty of Mathematical, Physical and Natural Sciences of Tunis, University of Tunis El Manar, 2092 Tunis, Tunisia. Electronic address: ibrahimchourouk@yahoo.fr.
2
National School of Veterinary Medicine at Sidi Thabet, 2020 Tunis, University of Manouba, Tunisia. Electronic address: saleehhammami@yahoo.fr.
3
Veterinary Research Institute of Tunisia, Laboratory of Virology, 1006, La Rabta, Tunis, Tunisia. Electronic address: selma_mejri@yahoo.fr.
4
Centre of Research and Water Technologies (CERTE), Laboratory of Treatment and Wastewater Valorisation, 8020, Techno Park of Borj-Cedria, Tunisia. Electronic address: ines_mehri@yahoo.fr.
5
National Reference Centre for Enteric Viruses, Laboratory of Virology, University Hospital of Dijon, 21070 Dijon, France. Electronic address: pierre.pothier@u-bourgogne.fr.
6
Centre of Research and Water Technologies (CERTE), Laboratory of Treatment and Wastewater Valorisation, 8020, Techno Park of Borj-Cedria, Tunisia. Electronic address: abdohas@gmail.com.

Abstract

Enteric viruses are released in important quantities into the environment where they can persist for a very long time. At very low doses, they can cause human gastroenteritis, and are responsible for a substantial number of waterborne diseases. The aims of this study were multiple: firstly, to study the circulation of Aichi viruses (AiV) in wastewater sampled at the scale of a pilot wastewater treatment plant; secondly, to evaluate the performance of two wastewater treatment procedures, as natural oxidizing lagoons and rotating Biodisks, concerning the AiV removal; and finally, to determine the different type of AiV genotype found during this study. Hence, the pilot wastewater treatment plant is principally irrigated by the wastewater of three neighbouring clinics. Wastewater samples were collected during 2011 from the two lines of biological treatment procedures. AiV detection in wastewater were achieved using the Reverse Transcription Polymerase Chain Reaction (RT-PCR) technique, and the identification of AiV genotype was realized by the direct sequencing of PCR products. The result revealed that AiV strains were identified in 50% (n = 51) of the wastewater samples. A significant increase of the AiV detection frequency was registered from upstream to downstream of the five ponds constituting the natural oxidizing lagoon process, and at the exit of the rotating Biodisks procedure. All detected AiV strains showed the highest nucleotide sequence identity to genotype B that has been recently observed in patients in Asia. This finding represented the first Tunisian survey that revealed and mentioned the first detection of AiV genotype B in sewage and by the same argued for a noticeable resistance or survival of this type of virus in the two lines of treatment considered.

KEYWORDS:

Aichi viruses; Genotype B; Natural oxidizing ponds; Rotating biodisks; Tunisia; Wastewater treatment

PMID:
28596124
DOI:
10.1016/j.micpath.2017.06.001
[Indexed for MEDLINE]

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