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Elife. 2017 May 2;6. pii: e24109. doi: 10.7554/eLife.24109.

Malaria parasite LIMP protein regulates sporozoite gliding motility and infectivity in mosquito and mammalian hosts.

Author information

1
Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Edifício Egas Moniz, Av. Prof. Egas Moniz, Lisbon, Portugal.
2
Parasitology, Department of Infectious Diseases, University of Heidelberg Medical School, Heidelberg, Germany.
3
Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, New Delhi, India.
4
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands.

Abstract

Gliding motility allows malaria parasites to migrate and invade tissues and cells in different hosts. It requires parasite surface proteins to provide attachment to host cells and extracellular matrices. Here, we identify the Plasmodium protein LIMP (the name refers to a gliding phenotype in the sporozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion during gliding motility in the rodent malaria model P. berghei. Transcribed in gametocytes, LIMP is translated in the ookinete from maternal mRNA, and later in the sporozoite. The absence of LIMP reduces initial mosquito infection by 50%, impedes salivary gland invasion 10-fold, and causes a complete absence of liver invasion as mutants fail to attach to host cells. GFP tagging of LIMP caused a limping defect during movement with reduced speed and transient curvature changes of the parasite. LIMP is an essential motility and invasion factor necessary for malaria transmission.

KEYWORDS:

Plasmodium berghei; cell adhesion; cell biology; gliding motility; infectious disease; malaria; microbiology; transmission

PMID:
28525314
PMCID:
PMC5438254
DOI:
10.7554/eLife.24109
[Indexed for MEDLINE]
Free PMC Article

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