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J Diabetes Complications. 2017 Jul;31(7):1103-1108. doi: 10.1016/j.jdiacomp.2017.04.024. Epub 2017 May 10.

Urinary lysophopholipids are increased in diabetic patients with nephropathy.

Author information

1
Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, Toulouse, France; Université Toulouse III Paul-Sabatier, Toulouse, France. Electronic address: jean-sebastien.saulnier-blache@inserm.fr.
2
CHU de Poitiers, Service d'Endocrinologie, Pole DUNE, 86021, Poitiers, France; Université de Poitiers, UFR Médecine Pharmacie, 86021, Poitiers, France; Inserm, CIC 1402 & U1082, 86021, Poitiers, France.
3
CHU de Tours, Service Néphrologie-immunologie clinique, 37000, Tours, France; Université François-Rabelais, EA4245, Inserm, 37000, Tours, France.
4
Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, Toulouse, France; Université Toulouse III Paul-Sabatier, Toulouse, France; CHU de Toulouse, Service de Diabétologie, Maladies Métaboliques et Nutrition, 31059, Toulouse, France.
5
Université Paris Diderot, Sorbonne Paris Cité, 75013, Paris, France; Inserm, UMRS1138, Centre de Recherche des Cordeliers, 75006, Paris, France, AP-HP; Hôpital Bichat, Diabétologie, Endocrinologie Nutrition, DHU FIRE, 75018, Paris, France.
6
Université de Lorraine & CHRU de Nancy, Diabétologie, Maladies Métaboliques et Nutrition, 54511, Vandoeuvre lès Nancy.
7
Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, Toulouse, France; Metatoul-Lipidomic core facility, MetaboHub, Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, 2 Université Toulouse III Paul-Sabatier, Toulouse, France.
8
Institut National de la Santé et de la Recherche Médicale (INSERM), U1188 - Université de La Réunion, France.
9
Institut National de la Santé et de la Recherche Médicale (INSERM), U1048, Institut of Cardiovascular and Metabolic Disease, Toulouse, France; Université Toulouse III Paul-Sabatier, Toulouse, France. Electronic address: joost-peter.schanstra@inserm.fr.

Abstract

Diabetic nephropathy (DN) is a major cause of chronic kidney disease that frequently leads to end stage renal failure. Lysophosphatidic acid (LPA) and lysophosphatidylcholine (LPC) are lysophospholipid mediators shown to accumulate in kidney and to promote renal inflammation and tubulo-interstitial fibrosis in diabetic rodent models. Here we assessed whether LPA and LPC were associated to the development of nephropathy in diabetic human patients. Several molecular species of LPA and LPC were quantified by LC/MS-MS in urine and plasma from type 2 diabetic patients with (cases; n=41) or without (controls, n=41) nephropathy symptoms (micro/macro-albuminuria and eGFR<60ml/min/1.73m2). Cases and controls were matched for sex, age and diabetes duration. Six species were detected in urine for both LPA and LPC, LPA16:0, LPA20:4, LPC16:0, LPC18:0, LPC18:1, and LPC18:2 that were significantly more concentrated in cases than in controls. Total LPC and LPA (sum of detected species) were significantly and exclusively associated with albuminuria (P<0.0001 and P=0.0009 respectively) and were significantly higher in the 3rd when compared to the 1st albuminuria tertile in cases. Plasma lysophospholipids showed a different species profile urine and their concentrations were not different between cases and controls. In conclusion, urine concentration of lysophospholipids increases in diabetic patients with DN as the likely result of their co-excretion with albumin combined with possible local production by kidney. Because LPA and LPC are known to promote renal inflammation and tubulo-interstitial fibrosis, their increased production in DN could participate to the development of kidney damage associated with diabetes.

KEYWORDS:

Diabetic nephropathy; Lysophosphatidic acid; Lysophosphatidylcholine; Urine

PMID:
28506691
DOI:
10.1016/j.jdiacomp.2017.04.024
[Indexed for MEDLINE]

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