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Mol Reprod Dev. 2017 Sep;84(9):814-821. doi: 10.1002/mrd.22829. Epub 2017 Jun 8.

Developmental competence of porcine genome-edited zygotes.

Author information

1
Department of Animal Medicine and Surgery, University of Murcia, Murcia, Spain.
2
Salk Institute for Biological Studies, La Jolla, California.
3
Department of Animal Science, UC Davis, Davis, California.

Abstract

Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases-including ZFNs, TALENs, and the CRISPR/Cas9 system-has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

KEYWORDS:

CRISPR/Cas9; TALEN; ZFN; cytoplasmic microinjection; in vitro-produced zygotes; in vivo-derived zygotes

PMID:
28471514
DOI:
10.1002/mrd.22829
[Indexed for MEDLINE]

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