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Genetica. 2017 Jun;145(3):275-293. doi: 10.1007/s10709-017-9964-z. Epub 2017 Apr 19.

Transposable elements in the Anopheles funestus transcriptome.

Author information

1
Fundação Oswaldo Cruz, Escola Nacional de Saúde Pública, Av. Brasil, 4365, Rio de Janeiro, Brazil. dfernandezmedina@gmail.com.
2
Departamento de Biologia, UNESP-Universidade Estadual Paulista, Rua Cristóvão Colombo, 2265, São José do Rio Preto, SP, Brazil.
3
Fundação Oswaldo Cruz, Escola Nacional de Saúde Pública, Av. Brasil, 4365, Rio de Janeiro, Brazil.
4
Laboratory of Malaria and Vector Research, NIAID/NIH, Rockville, MD, 20852, USA.

Abstract

Transposable elements (TEs) are present in most of the eukaryotic genomes and their impact on genome evolution is increasingly recognized. Although there is extensive information on the TEs present in several eukaryotic genomes, less is known about the expression of these elements at the transcriptome level. Here we present a detailed analysis regarding the expression of TEs in Anopheles funestus, the second most important vector of human malaria in Africa. Several transcriptionally active TE families belonging both to Class I and II were identified and characterized. Interestingly, we have identified a full-length putative active element (including the presence of full length TIRs in the genomic sequence) belonging to the hAT superfamily, which presents active members in other insect genomes. This work contributes to a comprehensive understanding of the landscape of transposable elements in A. funestus transcriptome. Our results reveal that TEs are abundant and diverse in the mosquito and that most of the TE families found in the genome are represented in the mosquito transcriptome, a fact that could indicate activity of these elements.The vast diversity of TEs expressed in A. funestus suggests that there is ongoing amplification of several families in this organism.

KEYWORDS:

Anopheles funestus; Transcriptome; Transposable elements

PMID:
28424974
PMCID:
PMC5584644
DOI:
10.1007/s10709-017-9964-z
[Indexed for MEDLINE]
Free PMC Article

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