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Biopreserv Biobank. 2017 Aug;15(4):305-309. doi: 10.1089/bio.2016.0105. Epub 2017 Feb 22.

Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview.

Author information

1
1 Spanish HIV HGM BioBank, Hospital General Universitario Gregorio Marañón , Instituto de Investigación Sanitaria Gregorio Marañón, Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain .
2
2 Biobanco Vasco para la Investigación-OEHUN , Vizcaya, Spain .
3
3 Instituto de Investigación Marqués de Valdecilla , Biobanco Valdecilla, Santander, Spain .
4
4 Biobanco Hospital Clínic-IDIBAPS , Institut d'Investigacions Biomèdiques August Pi i Sunyer, IDIBAPS, Barcelona, Spain .
5
5 Hospital Virgen de la Arrixaca , BIOBANC-MUR, Murcia, Spain .
6
6 Clínica Universidad de Navarra , Biobanco de la Universidad de Navarra, Pamplona, Spain .
7
7 Hospital Regional Universitario Carlos Haya , Málaga, Spain .
8
8 Centro de Investigación Biomédica en Red de Enfermedades Raras , CIBERER BIOBANK, Valencia, Spain .

Abstract

The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible interbiobank and intrabiobank assay variability.

KEYWORDS:

PBMCs; SOP; biobanks; cryopreservation; functionality

PMID:
28398808
DOI:
10.1089/bio.2016.0105
[Indexed for MEDLINE]

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