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Mediators Inflamm. 2017;2017:9474896. doi: 10.1155/2017/9474896. Epub 2017 Feb 21.

High Fat Diet Alters Gut Microbiota and the Expression of Paneth Cell-Antimicrobial Peptides Preceding Changes of Circulating Inflammatory Cytokines.

Author information

1
School of Pharmacy and Biological Engineering, Chengdu University, Sichuan, China; School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA, USA.
2
School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA, USA.
3
School of Pharmacy and Biological Engineering, Chengdu University, Sichuan, China.
4
Department of Food Science, University of Massachusetts, Amherst, MA, USA.
5
Grand Forks Human Nutrition Research Center, Agricultural Research Service, United States Department of Agriculture, Grand Forks, ND, USA.
6
School of Public Health and Health Sciences, University of Massachusetts, Amherst, MA, USA; Jean Mayer USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA.

Abstract

Obesity is an established risk factor for many diseases including intestinal cancer. One of the responsible mechanisms is the chronic inflammation driven by obesity. However, it remains to be defined whether diet-induced obesity exacerbates the intestinal inflammatory status by cytokines produced in adipose tissue or the high fat diet first alters the gut microbiota and then drives intestinal inflammation. To address this question, we fed C57BL/6 mice with a high fat diet (HF, 60%) and sacrificed them sequentially after 8, 12, and 16 weeks, and then compositions of gut microbiota and expressions of antimicrobial peptides were determined. The compositions of gut microbiota were altered at 8 wk HF feeding, followed with reduced Paneth antimicrobial peptides lysozyme and Reg IIIγ after 12 and 16 wk HF feeding (p < 0.05), whereas elevations of circulating inflammatory cytokines IFNγ and TNF-α were observed until feeding a HF diet for 16 weeks (p < 0.05). These results indicated that high fat diet may stimulate intestinal inflammation via altering gut microbiota, and it occurs prior to the potential influence by circulating inflammatory cytokines. These findings emphasized the importance of microbiota, in addition to adipose tissue per se, in driving intestinal inflammation, which may thereafter promote intestinal tumorigenesis.

PMID:
28316379
PMCID:
PMC5339499
DOI:
10.1155/2017/9474896
[Indexed for MEDLINE]
Free PMC Article

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