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Chembiochem. 2017 Jun 1;18(11):996-1000. doi: 10.1002/cbic.201700126. Epub 2017 May 3.

A Biocatalytic Nanomaterial for the Label-Free Detection of Virus-Like Particles.

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School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Gründenstrasse 40, 4132, Muttenz, Switzerland.
Laboratory of Virology, National Reference Center for Enteric Viruses, CHU F. Mitterrand, F and AgroSup Dijon, PAM UMR A 02.102, University of Burgundy-Franche-Comté, 26, Bd Docteur-Petitjean, 21079, Dijon, France.
INOFEA AG, Hochbergerstrasse 60C, 4057, Basel, Switzerland.
School of the Environment, Nanjing University, Xianlin Ave 63, 210093, Nanjing, China.


The design of nanomaterials that are capable of specific and sensitive biomolecular recognition is an on-going challenge in the chemical and biochemical sciences. A number of sophisticated artificial systems have been designed to specifically recognize a variety of targets. However, methods based on natural biomolecular detection systems using antibodies are often superior. Besides greater affinity and selectivity, antibodies can be easily coupled to enzymatic systems that act as signal amplifiers, thus permitting impressively low detection limits. The possibility to translate this concept to artificial recognition systems remains limited due to design incompatibilities. Here we describe the synthesis of a synthetic nanomaterial capable of specific biomolecular detection by using an internal biocatalytic colorimetric detection and amplification system. The design of this nanomaterial relies on the ability to accurately grow hybrid protein-organosilica layers at the surface of silica nanoparticles. The method allows for label-free detection and quantification of targets at picomolar concentrations.


biocatalysis; molecular recognition; nanoparticles; organosilica; viruses

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