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Leuk Res. 2017 Apr;55:91-96. doi: 10.1016/j.leukres.2017.01.020. Epub 2017 Jan 22.

The epigenetically-regulated miR-34a targeting c-SRC suppresses RAF/MEK/ERK signaling pathway in K-562 cells.

Author information

1
Department of Hematology, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Beijing 100042, China.
2
Beijing Institute of Radiation Medicine, Chinese PLA Academy of Military Medical Science, Beijing 100850, China.
3
Aerospace Balance Medical Center, Chinese PLA Air Force General Hospital, Beijing 100042, China.
4
Department of Hematology, Chinese PLA Air Force General Hospital, 30 Fucheng Road, Beijing 100042, China. Electronic address: wanghengxiang123@aliyun.com.

Abstract

Previous reports show that miR-34a suppressed K-562 cell proliferation and contributed to megakaryocytic differentiation of K-562 cells. Here, we reported that miR-34a, a tumor suppressor gene, is down-regulated in the K-562 cells and chronic myeloid leukemia (CML) patients due to aberrant DNA hypermethylation. c-SRC is a target of miR-34a. Restoring miR-34a expression resulted in down-regulation of c-SRC and phosphorylated (Tyr416) c-SRC protein in K-562 cells, which consequently triggered suppression of the RAF/MEK/ERK signaling pathway to decrease cell proliferation.

KEYWORDS:

Epigenetic; Methylation; c-SRC; miR-34a

PMID:
28157629
DOI:
10.1016/j.leukres.2017.01.020
[Indexed for MEDLINE]

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