Format

Send to

Choose Destination
Methods Mol Biol. 2017;1564:63-79. doi: 10.1007/978-1-4939-6813-8_7.

Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.

Author information

1
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011-3650, USA.
2
Department of Plant Pathology, Kansas State University, Manhattan, KS, 66506-5502, USA.
3
Harvard Medical School, Harvard University, Boston, MA, 02114-2790, USA.
4
Department of Agronomy, Iowa State University, Ames, IA, 50011-3650, USA.
5
Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011-3650, USA. yin@iastate.edu.

Abstract

Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.

KEYWORDS:

ChIP-seq; Gene expression; RNA-seq; Target genes; Transcription factor

PMID:
28124247
DOI:
10.1007/978-1-4939-6813-8_7
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center