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BMC Complement Altern Med. 2017 Jan 23;17(1):70. doi: 10.1186/s12906-017-1579-5.

Panaxatriol saponins promotes angiogenesis and enhances cerebral perfusion after ischemic stroke in rats.

Hui Z1,2,3,4,5, Sha DJ6,3,4,5, Wang SL2, Li CS1, Qian J6,4, Wang JQ2, Zhao Y2, Zhang JH2, Cheng HY7, Yang H1, Yu LJ7, Xu Y8,9,10,11,12,13.

Author information

1
Department of Neurology, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210008, Jiangsu, People's Republic of China.
2
Department of Neurology, The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, Jiangsu, People's Republic of China.
3
The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210008, Jiangsu, People's Republic of China.
4
Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, 210008, Jiangsu, People's Republic of China.
5
Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing, 210008, People's Republic of China.
6
Department of Emergency, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, 210008, Jiangsu, People's Republic of China.
7
Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, 321 ZhongShan Road, Nanjing City, Jiangsu Province, 210008, People's Republic of China.
8
Department of Neurology, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210008, Jiangsu, People's Republic of China. xuyun20042001@aliyun.com.
9
Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, 321 ZhongShan Road, Nanjing City, Jiangsu Province, 210008, People's Republic of China. xuyun20042001@aliyun.com.
10
The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210008, Jiangsu, People's Republic of China. xuyun20042001@aliyun.com.
11
Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, Nanjing, 210008, Jiangsu, People's Republic of China. xuyun20042001@aliyun.com.
12
Jiangsu Province Stroke Center for Diagnosis and Therapy, Nanjing, 210008, People's Republic of China. xuyun20042001@aliyun.com.
13
Nanjing Neuropsychiatry Clinic Medical Center, Nanjing, 210008, People's Republic of China. xuyun20042001@aliyun.com.

Abstract

BACKGROUND:

Panaxatriol saponins (PTS), an extract from the traditional Chinese herb Panax notoginseng, which has been used to treat ischemic stroke for many years in China. However, the mechanism underlying the effects of PTS remains unclear. This study aimed to determine whether PTS can protect against ischemic brain injury by promoting angiogenesis and to explore the possible mechanism by which it promotes angiogenesis.

METHODS:

Middle cerebral artery occlusion (MCAO) was induced in rats, and neurological deficit scores and brain infarct volumes were assessed. Micro-Positron emission tomography (PET) was adopted to assess cerebral perfusion, and real-time PCR and western blotting were used to evaluate vascular growth factor and Sonic hedgehog (Shh) pathway component levels. Immunofluorescence staining was used to determine capillary densities in ischemic penumbrae.

RESULTS:

We showed that PTS improved neurological function and reduced infarct volumes in MCAO rats. Micro-PET indicated that PTS can significantly increase 18F-fluorodeoxyglucose (18F-PDG) uptake by ischemic brain tissue and enhance cerebral perfusion after MCAO surgery. Moreover, PTS was able to increase capillary densities and enhance angiogenesis in ischemic boundary zones and up-regulate vascular endothelial growth factor (VEGF) and Angiopoietin-1 (Ang-1) expression by activating the Shh signaling pathway.

CONCLUSION:

These findings indicate that PTS exerts protective effects against cerebral ischemic injury by enhancing angiogenesis and improving microperfusion.

KEYWORDS:

Angiogenesis; Cerebral perfusion; Ischemic stroke; Panaxatriol saponins

PMID:
28114983
PMCID:
PMC5259846
DOI:
10.1186/s12906-017-1579-5
[Indexed for MEDLINE]
Free PMC Article

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