Format

Send to

Choose Destination
J Phys Chem B. 2017 Feb 16;121(6):1169-1175. doi: 10.1021/acs.jpcb.6b09021. Epub 2017 Feb 6.

Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags.

Author information

1
Department of Chemistry, Columbia University , New York, New York 10027, United States.
2
Department of Pure and Applied Sciences, University of Urbino , Loc. Crocicchia, 61029 Urbino, Italy.

Abstract

Dynamic nuclear polarization is an emerging technique for sensitizing solid-state NMR experiments by transferring polarization from electrons to nuclei. Stable biradicals, the polarization source for the cross effect mechanism, are typically codissolved at millimolar concentrations with proteins of interest. Here we describe the high-affinity biradical tag TMP-T, created by covalently linking trimethoprim, a nanomolar affinity ligand of dihydrofolate reductase (DHFR), to the biradical polarizing agent TOTAPOL. With TMP-T bound to DHFR, large enhancements of the protein spectrum are observed, comparable to when TOTAPOL is codissolved with the protein. In contrast to TOTAPOL, the tight binding TMP-T can be added stoichiometrically at radical concentrations orders of magnitude lower than in previously described preparations. Benefits of the reduced radical concentration include reduced spectral bleaching, reduced chemical perturbation of the sample, and the ability to selectively enhance signals for the protein of interest.

PMID:
28099013
PMCID:
PMC5518711
DOI:
10.1021/acs.jpcb.6b09021
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for American Chemical Society Icon for PubMed Central
Loading ...
Support Center