Caspase 3, 6, 8, and 9 Gene Expression in Peripheral Blood Leukocytes and Plasma Concentrations of IL-6 and TNF-α in Carriers of Different Polymorphic Marker -174G>C Genotypes of IL6 Gene Associated with the Risk of Nonalcoholic Steatohepatitis

I V Kurbatova; L V Topchieva; O P Dudanova

doi:10.1007/s10517-017-3618-0

Caspase 3, 6, 8, and 9 Gene Expression in Peripheral Blood Leukocytes and Plasma Concentrations of IL-6 and TNF-α in Carriers of Different Polymorphic Marker -174G>C Genotypes of IL6 Gene Associated with the Risk of Nonalcoholic Steatohepatitis

Bull Exp Biol Med. 2017 Jan;162(3):370-374. doi: 10.1007/s10517-017-3618-0. Epub 2017 Jan 14.

Authors

I V Kurbatova¹, L V Topchieva², O P Dudanova³

Affiliations

¹ Institute of Biology, Karelian Research Center, the Russian Academy of Sciences, Petrozavodsk, Russia. irina7m@yandex.ru.
² Institute of Biology, Karelian Research Center, the Russian Academy of Sciences, Petrozavodsk, Russia.
³ Petrozavodsk State University, Petrozavodsk, Russia.

PMID: 28091912
DOI: 10.1007/s10517-017-3618-0

Abstract

We revealed an association of IL6 gene -174G>C polymorphism with the development of nonalcoholic steatohepatitis in the Russian population. The risk is significantly higher in carriers of C allele: OR=1.77 (1.04; 3.02). The effects of -174G>C substitution in IL6 gene involving caspase 9 gene transcripts in peripheral blood leukocytes and on blood content of TNF-α in healthy individuals without clinical manifestations of nonalcoholic steatohepatitis were detected. The content of caspase 9 gene transcripts in peripheral blood leukocytes and plasma level of TNF-α were significantly higher in healthy subjects carrying C allele than in carriers of GG genotype. The levels of caspases 3, 6, 8, and 9 gene transcripts in peripheral blood leukocytes and plasma concentrations of TNF-α in patients with nonalcoholic steatohepatitis did not depend on IL6 genotype by -174G<C marker.

Keywords: caspases; interleukin-6; nonalcoholic steatohepatitis; polymorphism.

MeSH terms

Adult
Alleles
Caspase 3 / genetics
Caspase 3 / immunology
Caspase 6 / genetics
Caspase 6 / immunology
Caspase 8 / genetics
Caspase 8 / immunology
Caspase 9 / genetics*
Caspase 9 / immunology
Female
Gene Expression Regulation
Gene Frequency
Genetic Predisposition to Disease*
Heterozygote
Humans
Interleukin-6 / genetics*
Interleukin-6 / immunology
Leukocytes, Mononuclear / immunology
Leukocytes, Mononuclear / metabolism*
Leukocytes, Mononuclear / pathology
Male
Middle Aged
Non-alcoholic Fatty Liver Disease / diagnosis
Non-alcoholic Fatty Liver Disease / epidemiology
Non-alcoholic Fatty Liver Disease / genetics*
Non-alcoholic Fatty Liver Disease / immunology
Polymorphism, Single Nucleotide
Risk Factors
Russia / epidemiology
Signal Transduction
Tumor Necrosis Factor-alpha / genetics*
Tumor Necrosis Factor-alpha / immunology

Substances

IL6 protein, human
Interleukin-6
Tumor Necrosis Factor-alpha
CASP3 protein, human
CASP6 protein, human
CASP8 protein, human
CASP9 protein, human
Caspase 3
Caspase 6
Caspase 8
Caspase 9