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J Am Coll Cardiol. 2016 Dec 27;68(25):2819-2830. doi: 10.1016/j.jacc.2016.10.031.

Proteomics Research in Cardiovascular Medicine and Biomarker Discovery.

Author information

1
NIH BD2K Center of Excellence and Department of Physiology, Medicine and Bioinformatics, University of California, Los Angeles, California. Electronic address: magelpy@ucla.edu.
2
NIH BD2K Center of Excellence and Department of Physiology, Medicine and Bioinformatics, University of California, Los Angeles, California.
3
Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland. Electronic address: murphy1@mail.nih.gov.

Abstract

Proteomics is a systems physiology discipline to address the large-scale characterization of protein species within a biological system, be it a cell, a tissue, a body biofluid, an organism, or a cohort population. Building on advances from chemical analytical platforms (e.g., mass spectrometry and other technologies), proteomics approaches have contributed powerful applications in cardiovascular biomedicine, most notably in: 1) the discovery of circulating protein biomarkers of heart diseases from plasma samples; and 2) the identification of disease mechanisms and potential therapeutic targets in cardiovascular tissues, in both preclinical models and translational studies. Contemporary proteomics investigations offer powerful means to simultaneously examine tens of thousands of proteins in various samples, and understand their molecular phenotypes in health and disease. This concise review introduces study design considerations, example applications and use cases, as well as interpretation and analysis of proteomics data in cardiovascular biomedicine.

KEYWORDS:

mass spectrometry; molecular phenotyping; post-translational modifications; protein arrays; protein signatures; protein dynamics

PMID:
28007144
PMCID:
PMC5189682
DOI:
10.1016/j.jacc.2016.10.031
[Indexed for MEDLINE]
Free PMC Article

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