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Genes Dev. 2016 Dec 1;30(23):2565-2570. doi: 10.1101/gad.289553.116. Epub 2016 Dec 16.

Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.

Author information

1
Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
2
Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.
3
Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California at Los Angeles, Los Angeles, California 90095, USA.
4
Department of Pathology, Center for Precision Diagnostics, University of Washington, Seattle, Washington 98195, USA.
5
Institute for Advanced Biosciences (IAB), UMR5309, CNRS, U1209, Institut National de la Santé et de la Recherche Médicale (INSERM), Grenoble Alpes University, 38000 Grenoble, France.
6
Laboratoire de Biologie et Modélisation de la Cellule (LBMC), UMR 5239, CNRS/École Normale Supérieure de Lyon (ENSL)/Université Claude Bernard Lyon 1 (UCBL), 69007 Lyon, France.
7
Institut NeuroMyogène (INMG), UMR 5310, CNRS/UCBL/ENSL, 69007 Lyon, France.
8
Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.

Abstract

RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4-DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. We propose a model in which AGO4-DNA interaction could be responsible for the unique specificities of RdDM.

KEYWORDS:

Argonaute; DNA methylation; RdDM

PMID:
27986858
PMCID:
PMC5204349
DOI:
10.1101/gad.289553.116
[Indexed for MEDLINE]
Free PMC Article

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