Format

Send to

Choose Destination
Am J Physiol Renal Physiol. 2017 Mar 1;312(3):F533-F542. doi: 10.1152/ajprenal.00601.2016. Epub 2016 Dec 14.

From 20th century metabolic wall charts to 21st century systems biology: database of mammalian metabolic enzymes.

Author information

1
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; and.
2
Systems Biology Center, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
3
Epithelial Systems Biology Laboratory, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland; and knep@helix.nih.gov.

Abstract

The organization of the mammalian genome into gene subsets corresponding to specific functional classes has provided key tools for systems biology research. Here, we have created a web-accessible resource called the Mammalian Metabolic Enzyme Database (https://hpcwebapps.cit.nih.gov/ESBL/Database/MetabolicEnzymes/MetabolicEnzymeDatabase.html) keyed to the biochemical reactions represented on iconic metabolic pathway wall charts created in the previous century. Overall, we have mapped 1,647 genes to these pathways, representing ~7 percent of the protein-coding genome. To illustrate the use of the database, we apply it to the area of kidney physiology. In so doing, we have created an additional database (Database of Metabolic Enzymes in Kidney Tubule Segments: https://hpcwebapps.cit.nih.gov/ESBL/Database/MetabolicEnzymes/), mapping mRNA abundance measurements (mined from RNA-Seq studies) for all metabolic enzymes to each of 14 renal tubule segments. We carry out bioinformatics analysis of the enzyme expression pattern among renal tubule segments and mine various data sources to identify vasopressin-regulated metabolic enzymes in the renal collecting duct.

KEYWORDS:

collecting duct; kidney; metabolism; mitochondria; vasopressin

PMID:
27974320
PMCID:
PMC5374312
DOI:
10.1152/ajprenal.00601.2016
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center