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Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 2016 Nov 21.

Covalently circularized nanodiscs for studying membrane proteins and viral entry.

Author information

1
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
2
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
3
Department of Biochemistry, University of Zurich, Zurich, Switzerland.
4
Department of Chemistry and Institute for Advanced Study, Technische Universität München and Helmholtz Centre Munich, Munich, Germany.

Abstract

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

PMID:
27869813
PMCID:
PMC5199620
DOI:
10.1038/nmeth.4079
[Indexed for MEDLINE]
Free PMC Article

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