Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2017 Jan;14(1):49-52. doi: 10.1038/nmeth.4079. Epub 2016 Nov 21.

Covalently circularized nanodiscs for studying membrane proteins and viral entry.

Author information

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts, USA.
  • 2Department of Cell Biology, Harvard Medical School, Boston, Massachusetts, USA.
  • 3Department of Biochemistry, University of Zurich, Zurich, Switzerland.
  • 4Department of Chemistry and Institute for Advanced Study, Technische Universität München and Helmholtz Centre Munich, Munich, Germany.

Abstract

We engineered covalently circularized nanodiscs (cNDs) which, compared with standard nanodiscs, exhibit enhanced stability, defined diameter sizes and tunable shapes. Reconstitution into cNDs enhanced the quality of nuclear magnetic resonance spectra for both VDAC-1, a β-barrel membrane protein, and the G-protein-coupled receptor NTR1, an α-helical membrane protein. In addition, we used cNDs to visualize how simple, nonenveloped viruses translocate their genomes across membranes to initiate infection.

PMID:
27869813
PMCID:
PMC5199620
[Available on 2018-01-01]
DOI:
10.1038/nmeth.4079
[PubMed - in process]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Support Center